rhil 15 (R&D Systems)

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R&D Systems rhil 15
Rhil 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil+15/Recombinant+Human+IL-15+Protein/pmc13089343-228-54-57
Average 95 stars, based on 198 article reviews

rhil 15 - by Bioz Stars, 2026-09

95/100 stars

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Generated:

Article Title: PRDM15 inhibits responses of Tissue-Resident memory T cells by activating PVR/TIGIT axis to mediate gastric cancer immunosuppression.
Article Snippet: On day 2,1.25 ng/mL rhTGF-β1 (BioLegend) was added, and cells were cultured for an additional 2 days. .. For phenotype stability of TRM cells were generated as described, resuspended in media pre-equilibrated to 20% O2 with 10 ng/mL rhIL-15 (R&D Systems, Bio-Techne), with 1.25 ng/mL rhTGF-β1 (BioLegend)[17]. .. Tumour tissues were cut into 2-mm sized pieces and digested in 1 mg/ml collagenase type IV (BioFroxx, #2091MG100, CAS 9001-12-1), incubated at 37°C for 1 h, passed through 70-mm filter and suspended in PBS (0.5–1×106 cells/ml).

Cell Culture:

Article Title: Delivery of biomolecules to immune cells
Article Snippet: CD4+ T cells were separated from the CD14-negative fraction of PBMCs using CD14 and CD4 magnetic microbeads (MACS Miltenyi Biotec, Auburn, CA). .. T cells were cultured in RPMI 1640 media (Cellgro, Manassas, VA) containing 10% Human Serum (AB) (GemCell, West Sacramento, CA), 100 U/ml penicillin and streptomycin sulfate 100 μg/ml (H 10 medium) supplemented with 5 ng/ml rhIL-15 (R&D Systems, Minneapolis, MN) to maintain cell viability without cell activation. .. Human Monocyte derived Dendritic Cells (MDDCs) were prepared from CD14-positive monocytes selected from peripheral blood mononuclear cells using anti-CD14 magnetic microbeads (MACS Miltenyi Biotec) and cultured for 6 days with 100 ng/ml interleukin-4 and 50 ng/ml granulocyte-macrophage colony-stimulating factor (R & D Systems).

Activation Assay:

Immunopeptidomics:

Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination
Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, rhIL-21 (30 ng/ml; R&D Systems, 8879-IL) for 3 days in 48-well plate. rhIL-2 (30 IU/ml; R&D Systems, 202-IL), rhIL-7 (5 ng/ml; R&D Systems, 207-IL), and rhIL-15 (5 ng/ml; R&D Systems, 247-ILB) were supplemented every 2 days to the cocultured cells for a total duration of 14 days before the expanded T cells were used for immunogenicity validation assays. ..

Biomarker Discovery:

Incubation:

Article Title: Cytokine-induced memory-like natural killer cells in systemic lupus erythematosus patients.
Article Snippet: .. The MNCs (3 × 106/ ml) were incubated with rhIL-12 (10 ng/ml, Preprotech, Rocky Hill, USA), rhIL-15 (1 ng/ml), and IL-18 (50 ng/ ml, R&D Systems, USA) for 16 hours as the pre-activated group. .. Control and pre-activated cells were washed by PBS solution for 3 times, and cultured in RPMI-1640 with rhIL-15 (1 ng/ml) and 10% human AB serum (SigmaAldrich) for various time points.

Review